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From 2012.igem.org

Today's achievements:

1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before
2) Transformed bacteria spreaded on Petri's plate- LB-agar-ampiciline- incubation 37 degrees Celsius, overnight
3) SOC preparation:

      1,4g Hanahan's Broth
      0,18g Glucose
      50mL MiliQ water

filtrated on microfilter (if you have more important volume, autoclave it)