Team:SDU-Denmark/labwork/Notebook/week2
From 2012.igem.org
Laboratory Notebook
Here you find the log book for the procedures carried out in the laboratory, starting from week 27.
PCR of SST and FFT
We did a polymerase chain reaction with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers.
PCR program:
The genomic template we used was MCF7. The PCR samples were processed over night (O.N.).