Summary

The ever increasing complexity of genetic circuits will soon call for the need of enhanced biological data storage devices. In light of this we have devised an innovative generic memory system that couples to the signals in these circuits. This Cellular Logbook can remember which of these signals were encountered at what intensity or at which time. Storage is done by DNA methylation of restriction sites, after which we can read out whether or not a signal has been present by use of restriction enzymes and gel electrophoresis.

Furthermore, because of the dilution of methylated bits due to cellular proliferation and degradation, an indication of the time of registering can be inferred from the proportian of methylated bits in the total bits. This novel memory system not only offers great expandability in terms of detectable signals, but also holds great promise as a multi-sensor utility in experimental and industrial environments.

Model: Memory Generations