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Team:Macquarie Australia/Protocols/GibsonAssembly
From 2012.igem.org
Gibson Assembly Protocol
Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.
| Instruction | #1C | #2K | #2A | #3K | #3A | #4C | #5K | #5A |
|---|---|---|---|---|---|---|---|---|
| Addition of 20ng Gene Block Fragments in appropriate tube | 2µl Hemo_T7A | |||||||
| Heme oxygenase (T7 minus) | Hemo_A + Hemo_B + Plasmid | |||||||
| T7 Agrobacterium | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid | |||||||
| Agrobacterium (T7 minus) | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
    |
