Team:Macquarie Australia/Protocols/CompetentCellTesting

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Competent Cell Testing

Results:

  • The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg.

Recommendation:

  • New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg.
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