Team:LMU-Munich/Lab Notebook/Protocols
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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
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Protocols
On this page, we will upload unique protocols to share with the public.
Since we work with Bacillus subtilis as chassis we focus on protocols how to work with that organism.
1. Media and components for Bacillus subtilis
2. Transformation of Bacillus subtilis
3. Isolation of chromosomal DNA from Bacillus subtilis for transformation
4. https://static.igem.org/mediawiki/2012/6/6b/LMU-Munich_2012_Isolation_of_genomic_DNA_from_Bacillus_%28for_PCR....%29.pdf Isolation of chromosomal DNA from Bacillus subtilis (for PCR....)]
6. Clean deletion of genomic fragments in Bacillus subtilis
8. ß-Galactosidase Assay B. subtilis