Creating parts of Tar methylation region

Date:8/9

Colony PCR

→Reflection:We Took E.coli too many.You should take less E.coli.

→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)

Date:8/11

The purified DNA

→Reflection:Band was less.

PCR Product

Date:8/13

Confirmed of electrophoresis by PCR product and Ligation of the TA vector

Date:8/14

Transformation

Date:8/25

Miniprep

Creating parts of azurin

Date:8/16

Colony PCR

Ligation

Date:8/18

Colony PCR

Date:8/20

DNA extraction and purification of P.aeruginosa