Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see fragments which will be assembled using Gibson and fragment properties.
BioBrick name Part(s)
T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B
Heme oxygenase Plasmid Hemo_A Hemo_B
T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E
Agro Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E