Team:Cambridge/Diary/Week 10

From 2012.igem.org

Revision as of 09:17, 30 August 2012 by Emmyft (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This is a detailed record of our work during the Summer...Read More

Project

Outreach

Gallery

Diary

Team

Home

Safety

Sponsors

Week:	1	2	3	4	5	6	7	8	9	10	11	12

A few more people went on holiday and a few came back. Tom ran the gel for the lux-mOrange halves and nothing came out- he discovered that a few fragments we got out during the hectic Thursday and Friday are not of the right size, which might explain why our things are not working. That includes one part of the Ratiometrica vector backbone (vecB), and one part of the lux-mOrange backbone (vecA1). He re-PCRed these using touch-down PCR, and some of it seems to work now so we should start our Gibson again soon...

Tuesday

More PCR, more gels for Tom as he tries to get the correct fragments... He also grew up some overnight cultures with sfGFP-ampR plasmids and will miniprep so we now have more positive control templates.

Wednesday

Emmy did some reorganising and relabelling of all the tubes we've got as there are now too many tubes of DNA fragments for Ratiometrica and lux-mOrage! Tom did some more gel extractions and miniprepped the sfGFP-ampR out of the O/N cultures so now we have positive control again.