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Team:HokkaidoU Japan/Notebook/plastic Week 7
From 2012.igem.org
Contents |
August 16th
PCR
A PCR of pGEM to get linear PhaA and PhaB using Laury primer and X-PhaA-up-F, S-PhaA-dw-R, X-PhaB-up-F, S-PhaB-dw-R.
| pGEM(pGEM 1ul + DW 9ul) | 1 ul |
| up primer(10mM) | 1 ul |
| down primer(10mM) | 1 ul |
| 25mM MgSO4 | 3 ul |
| 2mM sNTPs | 5 ul |
| KOD plus neo | 1ul |
| 10xPCR buffer | 5 ul |
| DW | 33 ul |
PCR:60sec, 30 cycle 1h8m
Did not work well?
August 17th
Electrophoresis
Gel extraction
August 18th
Calculation of concentration for digestion
