Team:HokkaidoU Japan/Notebook/plastic Week 7

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August 17nd

PCR

A PCR of pGEM to get linear Pha A and PhaB using Laury primer.

　|KOD plus Neo 　|1ul

pGEM(pGEM 1ul + DW 9ul)	1 ul
up primer(10mM)	1 ul
down primer(10mM)	1 ul
25mM MgSO4	3ul
2mM sNTPs	5ul
10xPCR buffer	5 ul
DW	33 ul

PCR:60sec, 30 cycle 1h8m

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