Team:Leicester/Attributions

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iGEM Leicester Test Page 2012

Attributions

Each team must clearly attribute work done by the team on this page. They must distinguish work done by the team from work done by others, including the host labs, advisors, instructors, graduate students, and postgraduate masters students.

Christopher Morton

10th July: Plated out several of the CSE kits.
12th July: Photographed all of the CSE kits for recording the bacterial growth.
12th July: Helped with the testing of acetone on polystyrene.
12th July: Made the 1st plate of dissolved polystrene embedded in the Luria agar as a test to see if the method worked.
12th July: Made up dilutions of acetone and used methanol to see their effects on polystyrene.
13th July: Learnt how to use the grinder in an attempt to grind the polystyrene.
16th July: Prepared the minimal media.
16th July: Worked with the frozen polystyrene (-80oC) in an attempt to grind it down.
16th July: Worked with the liquid nitrogen in a further attempt to grind the polystyrene down.
18th July: Finished making the control, 5% and 10% polystyrene agar plates and took them to the other lab to be plated with ''Pseudomonas''.
19th July: Shaved EPS to try and recreate our suspension.
19th July: Plated up some more by sprinkling the polystyrene "sugar" on top of the agar.
23rd July: Went around town looking on the sponsorship drive.
31st July: Helped to produce the minimal media plates for the ''E. coli'' and ''Pseudomonas. sp'' as control plates.
31st July: Helped to produce the corning tubes for the liquid suspension of ''Pseudomonas sp''.
1st August: Produced the 15ml corning tubes for the suspension of ''Pseudomonas sp'' in rich media.
3rd August: Made the buffers B1 and B2 for the DNA extraction.
6th August - 10th August Genomic DNA Extraction with QIAGEN Maxi prep, Take one and two
13th August prepaired overnight cultures for the next Genomic tip (planned leave day)
14th August: finished off the second Genomic extraction, prepared the samples for the gel, worked out how to adapt the tips to allow for pressure to be applied
15rd August: loaded, ran and photographed the gel. started the preperatation for the next tip, made the gel for tomorrow

Anthony Cox

10th July: Plated out several of the CSE kits.
13th July: Used the mortar and pestle to try and grind the polystyrene.
13th July: Learnt how to use the grinder in an attempt to grind the polystyrene.
16th July: Prepared the minimal media.
16th July: Worked with the frozen polystyrene (-80oC) in an attempt to grind it down.
16th July: Worked with the liquid nitrogen in a further attempt to grind the polystyrene down.
18th July: Finished making the control, 5% and 10% polystyrene agar plates and took them to the other lab to be plated with ''Pseudomonas''.
19th July: Plated up some more by sprinkling the polystyrene "sugar" on top of the agar.
1st August: Made the protocol for the DNA hybridization.
2nd August: Made the protocol for the DNA extraction test, as well as the growth curves by diluting a broth.
3rd August: Did the CFU colonies and dilutions test with ''E. coli''.
7th August: Worked for the whole day doing spectrophotometry to finally get the experiment right and a good protocol.
8th August: Did the CFU colonies and dilutions test (again *sigh when will it be done right*) with ''E. coli''.

Philip Higgs

12th July: Photographed all of the CSE kits for recording the bacterial growth.
12th July: Helped with the testing of acetone on polystyrene.
12th July: Made the 1st plate of dissolved polystrene embedded in the Luria agar as a test to see if the method worked.
12th July: Made up dilutions of acetone and used methanol to see their effects on polystyrene.
13th July: Used the mortar and pestle to try and grind the polystyrene.
13th July: Learnt how to use the grinder in an attempt to grind the polystyrene.
16th July: Prepared the minimal media.
16th July: Worked with the frozen polystyrene (-80oC) in an attempt to grind it down.
16th July: Worked with the liquid nitrogen in a further attempt to grind the polystyrene down.
19th July: Spent almost the whole day researching methods of transport to Amsterdam and hotels for the stay at the Jamboree to find the best deal both pricewize and comfort.
20th July: Produced the nutrient broth for the liquid suspension plates.
23rd July: Went around town looking on the sponsorship drive.
31st July: Helped to produce the minimal media plates for the ''E. coli'' and ''Pseudomonas. sp'' as control plates.
31st July: Helped to produce the corning tubes for the liquid suspension of ''Pseudomonas sp''.
1st August: Sorted out all the information needed to pick the correct DNA extraction kit.
1st August: Produced the 15ml corning tubes for the suspension of ''Pseudomonas sp'' in rich media.
2nd August: Innoculated tshe 15ml corning tube with ''E. coli'' for the DNA extraction test.
3rd August: Did the CFU colonies and dilutions test with ''E. coli''.
6th August: Used the spectrophotomenter to give the DNA digest the starting value.
6th August: Innoculated the 3ml of luria broth for the final ''E. coli'' test.
7th August: Worked for the whole day doing spectrophotometry to finally get the experiment right and a good protocol.
8th August: Did the CFU colonies and dilutions test (again *sigh when will it be done right*) with ''E. coli''.
13th August: Plated out all the CSE kits onto 5% polystyrene on minimal agar.

William Harrison

10th July: Wrote the wiki entries for all of the plating and details.
16th July: Prepared the minimal media.
16th July: Worked with the liquid nitrogen in a further attempt to grind the polystyrene down.
18th July: Finished making the control, 5% and 10% polystyrene agar plates and took them to the other lab to be plated with ''Pseudomonas''.
19th July: Shaved EPS to try and recreate our suspension.
19th July: Plated up some more by sprinkling the polystyrene "sugar" on top of the agar.
20th July: Produced the nutrient broth for the liquid suspension plates.
23rd July: Filled out the COSHH form.
23rd July: Went around town looking on the sponsorship drive.
24th July: Filled out new COSHH form.
24th July: Called the sponsors found on the 23rd.
31st July: Helped to produce the minimal media plates for the ''E. coli'' and ''Pseudomonas. sp'' as control plates.
2nd August: Found all the ingredients needed for the experiments on the 3rd.
3rd August: Worked around with other labs to find all the ingredients for the buffers B1 and B2 for the DNA extraction test.
6th August - 10th August Genomic DNA Extraction with QIAGEN Maxi prep, Take one and two
14th August:finished off the second Genomic extraction, prepared the agerose and made the gel ready
15rd August: Nano drop spectrophotometry, started the preperatation for the next tip

Luke Thompson

9th July: Determined the protocol for plating out all the CSE kits coming back.
10th July: Plated out several of the CSE kits.
12th July: Helped with the testing of acetone on polystyrene.
12th July: Made up dilutions of acetone and used methanol to see their effects on polystyrene.
19th July: Made the method and tested it worked to sprinkle the polystyrene "sugar" on top of the agar.
3rd August: Made the buffers B1 and B2 for the DNA extraction.

Emily Halsey

12th July: Photographed all of the CSE kits for recording the bacterial growth.

Nathan Hanna

16th July: Wrote and edited the Rockethub script.
17th-31st July: Did all the editing/stitching together of the Rockethub video, as it was produced.
1st August: Tested the cooling for our hybridization in the DNA extraction and isolation processes.
3rd August: Did the CFU colonies and dilutions test with ''E. coli''.
20th August: Corrected the entire wiki, spelling errors, grammatical errors and punctuation errors.
 

Reema Naran

10th July: Plated out several of the CSE kits.

Mohammed Idres

10th July: Plated out several of the CSE kits.

Dr Badge

 

Dr Dalgleish

 

Dr Ketley

 

Dr Bayliss

 

Sue Hardy (Lab technician)

 
29th July: Plated up some ''E. coli'' for the team to use.
5th August: Produced the overnight cultures of ''E. coli'' for the team to use.

Alex (researcher)

 

Carlo (researcher)

16th July: Brought the team liquid nitrogen and showed us how to use it safely.

Pseudomonas researcher - Jaspreet Sahota

18th July: Plated out the control, 5% and 10% polystyrene plates with 5 strains of ''Pseudomonas''.

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