Team:Osaka/week3

August 12 (Sun)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Ligation
   • 153(C):1-2M(upstream)+1-10H(downstream)+1-1A(vector)
   • 154(C):1-22A(upstream)+1-2M(downstream)+1-1A(vector)
   • 155(C):1-2D(upstream)+1-23L(downstream)+1-1A(vector)
5. SDS-PAGE
   • Gel electrophoresis

August 16 (Thu)

1. Transformaton of ligation products into DH5α E.coli:153~155
2. Transformation of plasmid DNA into Rosetta E.coli:empty vector(A)

August 17 (Fri)

1. Transfer to liquid culture

August 18 (Sat)

1. Preparation of LB agar plates
2. Miniprep of yesterday's culture
3. PCR
4. Restriction digests of minipreppped parts
5. Gel electrophoresis of digests
6. PCR purification
7. Ligation

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