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Team:Macquarie/Protocols/Designing Gibson Assembly Fragments
From 2012.igem.org
Contents |
Haemoxygenase
- Original Gene Sequence: http://www.ncbi.nlm.nih.gov/nuccore/BA000022
Deinococcus radiodurans Bacteriophytochrome
- Original Gene Sequence: http://www.ncbi.nlm.nih.gov/nuccore/15807672/?from=53908&to=56175&strand=true&report=genbank
Agrobacterium tumefaciens Bacteriophytochrome
- Original Gene Sequence: http://www.ncbi.nlm.nih.gov/nuccore/159184118/?from=1954292&to=1956502&strand=true&report=genbank
BioBrick assembly using Gibson method: Fragment design
- Codon optimisation:
The Amino acid sequences for Haemoxygenase (synechocystis) and Bacteriophytochrome (Deinococcus radiodurans and Agrobacterium tumefaciens) were initially run through the Reverse Translate program (http://www.bioinformatics.org/sms2/rev_trans.html) for codon optimisation in E. coli BL21 .
