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From 2012.igem.org

March 19 - March 25

Thursday

• Expression plasmid pPMQAK1 (received from Sweden in February) was left growing.
• Brick J33210 was transformed in a broad host plasmid.
• New batch of competent E. coli (E. cloni) was prepared.
• Mipreps pPMQAK1 and pSB4K5
• Primers in for C1 and others (primers designed in meeting 1)
• Synechocystis PCC6803 culture was examined under confocal microscopy. The culture was not axenic.
• A designed construct was wrong. Included a template that was not in the kit 2011 (KanR + Double terminator, part K292003)
• Part K292003 was built by standard assembly (B1003+B1004)

No wet lab this week