Monday, August 13
To accurately verify that our construct is indeed producing zeaxanthin, spectrophotometry must be used to characterize our biobrick part. To this end, we tested crocin in two different solvents, along with our Z^2 construct, compared to the absorption spectrum of zeaxanthin in hexane.
http://dl.dropbox.com/u/88390549/CrocinGraphs.png This indicates that our Z^2 construct isn't actually producing crocin, or if it is, the crocin isn't expressed highly enough to absorb significantly.
We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded. Tuesday, August 14
Wednesday, August 15
Thursday, August 16
Friday, August 17
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