Gel extraction of DNA

Generation of Chemically Competent Cells

The protocol for this technique be found here and MSDS for the reagents used can be found here.

Risks

SYBR Safe - Since the agarose gel has been dyed with SYBR Safe, everything that could have come into contact with it needs to be treated as liquid hazardous waste, including gloves (which must be worn at all times during the whole procedure).