Transformation of E.coli by Electroporation

The protocol for this technique can be found here and MSDS for the reagents used can be found here.

Risks

Bacteria- Depending on the bacteria used in your experiments, the danger associated with exposure will vary. Our laboratory is only authorised to use Biosafety level 1 (non-pathogenic) bacteria and so in our experiments the risk to scientists is minimal. It is however the policy of the department to treat all bacteria as pathogens. This will be increasingly important if pathogenic strains of bacteria are used.

Infection - the risk of infection in our experiment was not great as non-pathogenic strains were used. Gloves and lab coats were still always worn when working with bacteria (this was also to help prevent contamination of samples). Where there is a greater risk masks should also be work to prevent access via the mouth or airways.

Despite wearing gloves while working with bacteria, scientists must still wash their hands before leaving the lab to eliminate the chance of contaminating food.

Disposal - Unused bacteria and any material that has come into contact with the bacteria (including gloves)should be disposed of in the biological waste vessels in the laboratory. These materials are then autoclaved and disposed of in accordance with normal departmental procedures.

Apparatus voltage - Though none of the reagents used are particularly harmful, the Genepulse device administers a very high voltage and therefore the electroporation cuvette should only be used with the device in the manner described by the manufacturer. This will normally involve the use of a plastic loading device which connects the cuvette to the electrodes prior to electroporation. If in doubt, seek advice before connecting the cuvette to the device.