1. Trash out the waste on the right bottom of the machine before you start.

2. Add bleach (detergent) to the tubes on the right positions.

3. Run 'Cytosoft 5.3'

4. Click Clean and Shut Down -> This would take around 15 min.

5. After cleaning, click Guava Express Plus on the left column.

6. Go to the Analysis and Click 'Open Data Set' to set up the condition as our previous experiments.

7. Go to folder 'iGEM' and open 'setting' file.

8. Go to Acquisition - and hold here.

9. Go to the desktop and run WorkEdit 5.3.

10. Highlight target sample areas.

11. Make the condition as Guava Express Plus and check the box of '3 sec' and setup the speed from high to medium.

12. Save and go back to the Cytosoft 5.3.

13. Start the worklist on the 'Go to Acquisition'.

14. Locate the 96 well on the machine.

15. Name our file as 'Today's date_title'

16. When you are asked to adjust setting, check one circle of the 96 well for the control (Sowmya will give seed control).

17. Compare with worklist and make sure that the conditions are the same as we made.

18. After completing the adjust setting, click 'Resume' button.

19. Wait around 10~15min till the end - data will be saved automatically.

20. After finishing the experiment, take out the 96 well and change the bleach position for the cleaning mode.

21. Go to Main menu and click 'clean and shut down'.

22. Now you done :)