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Contents 1 Columbia Copper Experiment Notebook 1.1 Copper Experiment 1 1.1.1 Friday, June 22nd 1.1.2 Saturday, June 23 1.1.3 Sunday, June 24 1.2 July, 2012

Columbia Copper Experiment Notebook

Copper Experiment 1

Friday, June 22nd

Outline of activity today:

• Met with Jason, who showed us how to make A. ferrooxidans liquid media, prepared Copper 1 and 2 samples (with and without bacteria) and placed on shaker. Will be periodically checked until June 25 twice a day for activity.
• Showed us recipe for solid media, and made enough for two samples, 20 mL (one control with no bacteria). Copper samples were submerged in agarose (see Steven’s pictures). Also learned protocols listed below.

Starting Sample masses:

Copper 1: (liquid media only)

Sample	Mass (g)
1	0.0092
2	0.0107.

Copper 2: (liquid media only)

Sample	Mass (g)
1	0.1318
2	0.1286.

Copper 2: (solid media)

Sample	Mass (g)
1	0.1272
2	0.1282.

Liquid media and flasks were placed in shaker at 11:35 am, 225 rpm, 30C. Solid media and petri dishes were placed in incubator at 12:42 pm, 30C.

Saturday, June 23

• Checked the progress of all copper samples that included Copper 2 samples in solid media with and without bacteria, Copper 1 and 2 samples in liquid media each containing two samples of with and without bacteria.
• Observations were made for all samples. Optical density was measured for all of the samples submerged in liquid basal AFM low pH solution. Mass was weighed only copper 2 in liquid media with and without bacteria.

Solid Media Samples:

Copper 2- Sample 1 (fully submerged, without bacteria)

• Observations: Solid media mostly clear except for the outer edges of the Copper which appeared to be a light golden yellow color (note picture below). Copper turned to a dark brown color. No observable size change to the copper.

Copper 2- Sample 2 (half submerged, with bacteria)

• Observations: Solid media turned from clear to a light golden yellow color (similar to that of the outer edges of the Copper from sample 1 mentioned above). Copper turned to a dark brown color. No observable size change to the copper.

Liquid Media Samples:

Copper 1- Sample 1 (with bacteria)

• Observations: Noticeable 7+ small pieces of copper sample within liquid media (note picture below). Conclude to not take mass for this sample.
• Cuvette reading2: 0.003 A
• Current mass: N/A, Previous mass: 0.0092g, Initial mass: 0.0092g
•  % Mass Lost: N/A

Copper 1- Sample 2 (without bateria)

• Observations: No significant copper pieces visible in liquid media (note picture). Conclude to not take mass for this sample.
• Cuvette reading: 0.004 A
• Current mass reading: N/A, Previous mass: 0.0107g, Initial mass: 0.0107g
•  % Mass lost: N/A

Copper 2- Sample 1 (with bacteria)

• Observations: No observable change (see photo below).
• Cuvette reading: .004A
• Current mass: 0.1063g, Previous mass: 0.1318g, Initial mass: 0.1318g
•  % Mass Lost: 19.35%

Copper 2- Sample 2 (without bacteria)

• Observations: No observable change to copper (see picture below).
• Cuvette reading: 0.040 A**
• Cuvette reading: 0.018 A***
• Current mass reading: 0.0953g, Previous mass: 0.1286g, Initial mass: 0.1286g*
•  % Mass lost: 25.89%

Sunday, June 24

• Took out all four samples for standard check of OD and mass of copper 2 samples. Solid media was also examined. All samples (liquid and solid) were photographed.
• No visible copper 1 samples present in either sample (see Observations)

Solid Media Samples: Copper 2- Sample 1 (fully submerged, without bacteria)

• Observations: A tint of green color on copper surface, the immediate radius of the copper has brown color, outside of the previously mentioned radius is a yellow tinge to the solid media. The rest of the solid media was clear. No observable size change.

Copper 2- Sample 2 (half submerged, with bacteria)

• Observations: A darker more present shade of green on copper, compared to that of sample 1 mentioned above. Outer edges of the copper appeared black. Media was a uniformed amber color. No observable size change.

Liquid Media Samples:

Copper 1- Sample 1 (with bacteria)

• Observations: Liquid media copper brown, that may include ions that are not copper. No visible copper pieces, and conclude to not take mass for this sample. During procedure, centrifuged for another 1 minute to original 2 minutes at sane speed (due to the lack of expected visible pellets of some kind*)
• Cuvette reading1: 0.025 A
• Current mass: N/A, Previous mass: N/A, Initial mass: 0.0092g
•  % Mass Lost: N/A

Copper 1- Sample 2 (without bateria)

• Observations: Slight light green color to the media, and media appeared slightly cloudy. No significant copper pieces visible in liquid media. Conclude to not take mass for this sample.
• Cuvette reading: 0.033 A
• Current mass reading: N/A, Previous mass: N/A, Initial mass: 0.0107g
•  % Mass lost: N/A

Copper 2- Sample 1 (with bacteria)

• Observations: Visible brown copper color to liquid media. Pellets formed when the media was centrifuged. Many fluctuations in mass was seen, possible sources of error maybe water droplets or cleanness of the tweezers.
• Cuvette reading: .667A
• Current mass 1: 0.0550g, Current mass 2: 0.0388g, Current mass 3: 0.0285g
• Previous mass: 0.1063g, Initial mass: 0.1318g
•  % Mass Lost 1: 48.26%,  % Mass Lost 2: 63.50%, % Mass Lost 3: 73.19%

Copper 2- Sample 2 (without bacteria)

• Observations: Copper is visible in faint blue-green liquid media. No observable change to copper. Observable pellets after centrifuging (Unknown origin). Note the varied measurements in the masses, although not of a significant drop as in Copper 2 liquid media sample 1.
• Cuvette reading: 0.040 A
• Current mass reading1: 0.0907g, Current mass reading2: .0842g
• Previous mass: 0.0953g, Initial mass: 0.1286g
•  % Mass lost 1: 4.83%, % Mass lost 2: 11.65%

July, 2012