Team:Evry/Notebook/w9

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Weeks:

June July August September October November

Week 9: 6th August - 12th August

Monday, 6th August

Reporter test

We had a lot of reporters BB but we didn't know if they're all working. We test them with a electrophoresis: Two RFP, a GFP, a CFP and a YFP worked.

GFP BB

Digestion of GFP BB with EcoRI and PstI, gel extraction and DNA purification. Goal: insertion in pCS2+ BB Issue: Not enough DNA for ligation. Mini-prep pCS2+-dendra2 and pCS2+-mcherry checked by gel. inolation for midi-prep pNHK60 (Tir1/GFP-aid), inoculation for mini-prep pSB1C3 K515100 (IaaH+IaaM).

Tuesday, 7th August

Midi-prep pNHK60 (Tir1/GFP-aid), Mini-prep pSB1C3 K515100 (IaaH+IaaM). Digestion pSB1C3 K515100 (IaaH+IaaM)with EcoRI and PstI then transformation in pCS2+ (amplified with PCR)

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