Team:Cambridge/Protocols

• PCR using Phusion DNA polymerase A method for amplifying a section of DNA.
• Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
• Gel Electrophoresis A technique for separating DNA strands of different lengths.
• Gel Extraction of DNA A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis.
• Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.
• Transformation of Escherichia coli A method for transforming competent E.coli with DNA
• Transformation of Bacillus subtilis A technique used to introduce foreign DNA into competent Bacillus cells.
• MiniPrep A method used to extract DNA from bacterial cells.
• Restriction Enzyme Digest A method for creating a restriction map of a plasmid.
• Protein analysis by SDS PAGE A method used to separate polypeptides of different lengths.
• Preparation of LB Agar Plates A method used to prepare agar plate to culture common bacteria.

N.B. Information in purple is subject to change through optimisation over the course of our project.

Lab supplies: [http://www.bioc.cam.ac.uk/stores/ BioPath Stores]