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Columbia Copper Experiment Notebook

Copper Experiment 1

Outline of activity today:

Met with Jason, who showed us how to make A. ferrooxidans liquid media, prepared Copper 1 and 2 samples (with and without bacteria) and placed on shaker. Will be periodically checked until June 25 twice a day for activity.
Showed us recipe for solid media, and made enough for two samples, 20 mL (one control with no bacteria). Copper samples were submerged in agarose (see Steven’s pictures). Also learned protocols listed below.

Starting Sample masses:

**Copper 1: (liquid media only)**
Sample	Mass (g)
1	0.0092
2	0.0107.

**Copper 2: (liquid media only)**
Sample	Mass (g)
1	0.1318
2	0.1286.

**Copper 2: (solid media)**
Sample	Mass (g)
1	0.1272
2	0.1282.

Liquid media and flasks were placed in shaker at 11:35 am, 225 rpm, 30C. Solid media and petri dishes were placed in incubator at 12:42 pm, 30C.

Checked the progress of all copper samples that included Copper 2 samples in solid media with and without bacteria, Copper 1 and 2 samples in liquid media each containing two samples of with and without bacteria.
Observations were made for all samples. Optical density was measured for all of the samples submerged in liquid basal AFM low pH solution. Mass was weighed only copper 2 in liquid media with and without bacteria.

Solid Media Samples:

Copper 2- Sample 1 (fully submerged, without bacteria)

Observations: Solid media mostly clear except for the outer edges of the Copper which appeared to be a light golden yellow color (note picture below). Copper turned to a dark brown color. No observable size change to the copper.

Copper 2- Sample 2 (half submerged, with bacteria)

Observations: Solid media turned from clear to a light golden yellow color (similar to that of the outer edges of the Copper from sample 1 mentioned above). Copper turned to a dark brown color. No observable size change to the copper.

Liquid Media Samples:

Copper 2- Sample 1 (with bacteria)

Copper 1- Sample 1 (with bacteria)

Observations: Noticeable 7+ small pieces of copper sample within liquid media (note picture below). Conclude to not take mass for this sample.
Cuvette reading2: 0.003 A
Current mass: N/A, Previous mass: 0.0092g, Initial mass: 0.0092g
% Mass Lost: N/A

Copper 1- Sample 2 (without bateria)

Observations: No significant copper pieces visible in liquid media (note picture). Conclude to not take mass for this sample.
Cuvette reading: 0.004 A
Current mass reading: N/A, Previous mass: 0.0107g, Initial mass: 0.0107g
% Mass lost: N/A

Copper 2- Sample 2 (without bacteria)