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Team:Carnegie Mellon
From 2012.igem.org
Revision as of 19:07, 22 May 2012 by JSalazar88 (Talk | contribs)
Team:Carnegie_Mellon
From 2012.igem.org
Contents |
Introduction: Motivation
- We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
- We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
- We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.
Place abstract/info here
Motivation question?
Humanistic implications go here
Primary Objective: A Useful BioBrick for Synthetic Biologists
We have been xxxxxyyyy for the purpose of xxxyyy which is detailed by the following:
- something great
- something greater
- something awesome
This will be xxxyyy
Secondary Objective: Secondary Objective
We have also been continuing the work of our 2007 team in engineering Escherichia coli to produce the vitamin A precursor β-carotene. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (WHO).
Further Considerations
In the pursuit of our project, as well as the biological aspects, we:
- considered aspects of scale-up, including the ethical, legal and social implications of our potential final product, MicroMaize,
- programmed a new piece of software for use in metabolic modelling,
- developed and tested a number of new techniques to make the process of creating BioBricksTM that little bit easier,
- collaborated with the University of Guelph team, sharing carotenoid synthesis genes, flux modulators, and gram positive plasmids.
