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Team:Cambridge/Diary/Week 6
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Contents |
Monday
We are suffering from some setback on the PCR front. Tom and Emmy's PCR re-runs for the large vectors did not work despite we lengthened the elongation time to 5 minutes per cycle. After sequence alignments we discovered that the primers may anneal to various sites on the primer, producing a range of undesired products. We will carry out touch down PCR tomorrow to make the annealing conditions more strigent.
PCR of the mOrange gene last Friday also yielded no visible products. Looking at the primers' designs again, we think it might be due to problems with the annealing temperature. Therefore we are setting PCR reactions across a temperature gradient today to find the optimum annealing temperature.
Tuesday
Gel electrophoresis of the mOrange PCR products give no visible bands. There also seem to be problem with our positive and negative controls.
Tuesday
Wednesday
Thursday
Friday
