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Team:EPF-Lausanne/Sequencing/LovTAP T7 27 07 2012
From 2012.igem.org
Revision as of 09:32, 31 July 2012 by Diego.marcos (Talk | contribs)
Compared with the plasmid sequence
Seq_2 is the plasmid we thought we had, and Seq_2 is the sequencing result using the T7 primer:
Alignment of Sequence_1: [Sequence Window #2] with Sequence_2: [Untitled Sequence #1]
Similarity : 1005/3216 (31.25 %)
Seq_1 1 ------------------------------------------------------------ 0
Seq_2 1 CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTC 60
Seq_1 1 ------------------------------------------------------------ 0
Seq_2 61 ATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGA 120
Seq_1 1 ------------------------------------------------------------ 0
Seq_2 121 GATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTT 180
Seq_1 1 ------------------------------------------------------------ 0
Seq_2 181 GGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGT 240
Seq_1 1 ------------------------------------------------------------ 0
Seq_2 241 GCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACG 300
Seq_1 1 ------------------------------------------------------------ 0
Seq_2 301 ACGGCCAGTGAGCGCGACGTAATACGACTCACTATAGGGCGAATTGGCGGAAGGCCGTCA 360
Seq_1 1 ---------------------GCTCCCAGCTTGGGCGCCACCATGGGCTTTACACCTCAC 39
| || |||||||||||||||||||||||||||||||||
Seq_2 361 AGGCCTAGGCGCGCCATGAGCTCCCCAAGCTTGGGCGCCACCATGGGCTTTACACCTCAC 420
Seq_1 40 GATTCTGCCCCTTACGGGGCCCTGGATATGGCCGACTTCGAGTTTGAGCAGATGTTCACC 99
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 421 GATTCTGCCCCTTACGGGGCCCTGGATATGGCCGACTTCGAGTTTGAGCAGATGTTCACC 480
Seq_1 100 GATGCTCTGGGGATCGACGAATACGGAGGACCAAAGAAAAAACGGAAAGTGGATATGCTG 159
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 481 GATGCTCTGGGGATCGACGAATACGGAGGACCAAAGAAAAAACGGAAAGTGGATATGCTG 540
Seq_1 160 GCCACAACACTGGAGAGAATTGAGAAAAACTTTGTGATCACCGACCCTCGACTGCCCGAT 219
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 541 GCCACAACACTGGAGAGAATTGAGAAAAACTTTGTGATCACCGACCCTCGACTGCCCGAT 600
Seq_1 220 AATCCCATCATTTTCGCTTCCGATTCCTTCCTCCAGCTCACTGAGTACTCTAGAGAGGAG 279
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 601 AATCCCATCATTTTCGCTTCCGATTCCTTCCTCCAGCTCACTGAGTACTCTAGAGAGGAG 660
Seq_1 280 ATTCTGGGCCGGAATTGTCGCTTCCTCCAGGGACCTGAAACCGATAGAGCCACTGTGAGA 339
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 661 ATTCTGGGCCGGAATTGTCGCTTCCTCCAGGGACCTGAAACCGATAGAGCCACTGTGAGA 720
Seq_1 340 AAAATCCGGGACGCCATCGATAATCAGACCGAGGTCACCGTCCAGCTCATCAACTACACC 399
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 721 AAAATCCGGGACGCCATCGATAATCAGACCGAGGTCACCGTCCAGCTCATCAACTACACC 780
Seq_1 400 AAATCTGGCAAAAAATTCTGGAACCTGTTCCACCTCCAGCCTATGAGAGATCAGAAGGGC 459
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 781 AAATCTGGCAAAAAATTCTGGAACCTGTTCCACCTCCAGCCTATGAGAGATCAGAAGGGC 840
Seq_1 460 GACGTCCAGTACTTTATTGGAGTCCAGCTGGATGGCACTGAACATGTGAGGGATGCTGCT 519
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 841 GACGTCCAGTACTTTATTGGAGTCCAGCTGGATGGCACTGAACATGTGAGGGATGCTGCT 900
Seq_1 520 GAACGAGAGGGCGTGATGCTCATCAAAAAAACCGCCGAGAATATCGACGAGGCCGCTTTT 579
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 901 GAACGAGAGGGCGTGATGCTCATCAAAAAAACCGCCGAGAATATCGACGAGGCCGCTTTT 960
Seq_1 580 GTGGACCTCCTGAAAAACGCTTACCAGAACGACCTCCACCTGCCACTCCTCAATCTCATG 639
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 961 GTGGACCTCCTGAAAAACGCTTACCAGAACGACCTCCACCTGCCACTCCTCAATCTCATG 1020
Seq_1 640 CTCACTCCCGATGAACGGGAAGCACTGGGCACACGAGTCCGAATTGTGGAGGAGCTGCTC 699
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 1021 CTCACTCCCGATGAACGGGAAGCACTGGGCACACGAGTCCGAATTGTGGAGGAGCTGCTC 1080
Seq_1 700 CGAGGCGAAATGTCTCAGAGAGAGCTGAAAAACGAACTGGGAGCCGGAATTGCTACCATT 759
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 1081 CGAGGCGAAATGTCTCAGAGAGAGCTGAAAAACGAACTGGGAGCCGGAATTGCTACCATT 1140
Seq_1 760 ACACGGGGATCCAACTCCCTGAAAGCCGCCCCTGTGGAACTCCGGCAGTGGCTCGAGGAG 819
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 1141 ACACGGGGATCCAACTCCCTGAAAGCCGCCCCTGTGGAACTCCGGCAGTGGCTCGAGGAG 1200
Seq_1 820 GTCCTGCTCAAATCCGATTGAGGAATTCCGGTACCTCTTAATTAACTGGCCTCATGGGCC 879
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 1201 GTCCTGCTCAAATCCGATTGAGGAATTCCGGTACCTCTTAATTAACTGGCCTCATGGGCC 1260
Seq_1 880 TTCCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAACATGGT 939
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 1261 TTCCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAACATGGT 1320
Seq_1 940 CATAGCTGTTTCCTTGCGTATTGGGCGCTCTCCGCTTCCTCGCTCACTGACTCGCTGCGC 999
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Seq_2 1321 CATAGCTGTTTCCTTGCGTATTGGGCGCTCTCCGCTTCCTCGCTCACTGACTCGCTGCGC 1380
Seq_1 1000 TCGGTCGTT--------------------------------------------------- 1008
|||||||||
Seq_2 1381 TCGGTCGTTCGGGTAAAGCCTGGGGTGCCTAATGAGCAAAAGGCCAGCAAAAGGCCAGGA 1440
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1441 ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC 1500
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1501 ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG 1560
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1561 CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT 1620
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1621 ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT 1680
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1681 ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC 1740
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1741 AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG 1800
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1801 ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG 1860
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1861 GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTG 1920
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1921 GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG 1980
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 1981 GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA 2040
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2041 GAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA 2100
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2101 ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA 2160
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2161 TCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGT 2220
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2221 CTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTT 2280
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2281 CATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCAT 2340
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2341 CTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAG 2400
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2401 CAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCT 2460
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2461 CCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTT 2520
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2521 TGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGG 2580
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2581 CTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCA 2640
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2641 AAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGT 2700
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2701 TATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGAT 2760
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2761 GCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGAC 2820
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2821 CGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAA 2880
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2881 AAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGT 2940
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 2941 TGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTT 3000
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 3001 TCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAA 3060
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 3061 GGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTT 3120
Seq_1 1009 ------------------------------------------------------------ 1008
Seq_2 3121 ATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAA 3180
Seq_1 1009 ------------------------------------ 1008
Seq_2 3181 TAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC 3216
Compared with just LovTAP
99.6% identity in 279 residues overlap; Score: 1426.0; Gap frequency: 0.0%
UserSeq1, 1 APSLGATMGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGGPKKKRKVDMLATTLERI
Lovtap, 2 SPSLGATMGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGGPKKKRKVDMLATTLERI
***********************************************************
UserSeq1, 61 EKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAID
Lovtap, 62 EKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAID
************************************************************
UserSeq1, 121 NQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVML
Lovtap, 122 NQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVML
************************************************************
UserSeq1, 181 IKKTAENIDEAAFVDLLKNAYQNDLHLPLLNLMLTPDEREALGTRVRIVEELLRGEMSQR
Lovtap, 182 IKKTAENIDEAAFVDLLKNAYQNDLHLPLLNLMLTPDEREALGTRVRIVEELLRGEMSQR
************************************************************
UserSeq1, 241 ELKNELGAGIATITRGSNSLKAAPVELRQWLEEVLLKSD
Lovtap, 242 ELKNELGAGIATITRGSNSLKAAPVELRQWLEEVLLKSD
***************************************
