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Protocol: Transfection CHO
- Cell Density 3x10^6cells/mL
- Total Volume 5 mL
- DNA 1.5 µg/10^6cells
- PEI 3 µg/10^6cells
- Passage seed 1 day prior to transfection
- Prepare tubes by addition of DNA
- Centrifuge the necessary volume of seed, remove conditioned medium and resuspend in necessary volume of fresh medium to acheive the required cell density
- Add 5 mL of the cell suspension to the tube with DNA and mix by orbitally shaking
- Add the PEI to the Cell+DNA mixture as soon as possible
- Place in the incubator at 37°C
