Transfection Protocol -> CHO-DG44

• Cell Density 3x10^6cells/mL
• Total Volume 5 mL
• DNA 1.5 µg/10^6cells
• PEI 3 µg/10^6cells

1. Passage seed 1 day prior to transfection
2. Prepare tubes by addition of DNA
3. Centrifuge the necessary volume of seed, remove conditioned medium and resuspend in necessary volume of fresh medium to acheive the required cell density

1. Add 5 mL of the cell suspension to the tube with DNA and mix by orbitally shaking
2. Add the PEI to the Cell+DNA mixture as soon as possible
3. Place in the incubator at 37°C