Microsynth [http://microsynth.ch Microsynth] is a Swiss sequencing company that has a pick-up service at EPFL, which means the sample doesn't need to be sent anywhere. It also has a [http://microsynth.ch/de/10190/Hints-and-Tips.html#stpl library of standard primers], in case there is some kind of common sequence in the vicinity of the sequence of interest that could be used as the starting point for Sanger sequencing.

Requirements 1. Design the sequencing primers that should start approximately 50 bp upstream and downstream of your sequence of interest. If there is a standard sequence in that area, pick a pre-made primer from the standard list. 2. Test your primers by running a virtual PCR with [http://serialbasics.free.fr/Serial_Cloner.html Serial Cloner] on your sequence.