Monday, July 23
Today we sonicated three cultures of E. coli - one with our Z construct, one with Z, C and inducers, and one with Z, C, but no inducer. Then, we extracted the soluble proteins from those cultures and ran them on a gel. [PICTURE]
We also ran a PCR of a series of ligations. The color constructs were ligated with promoters into chloramphenicol-resistant plasmid Psb1C3. [EXPAND UPON THIS]
Extracted proteins from cultures and took spectrophotometric readings - show graphs
Tuesday, July 24
Ordered Primers
Ligation of RFP construct with PSL2131
Wednesday, July 25
We have discovered that we need a fusion protein in order to deal with insoluble protein products that form inclusion bodies, preventing the effective transcription of our gene. Thus, we obtained ________ from the lab of James Havranek.
Thursday, July 26
Friday, July 27
Friday, July 28
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