Team:Colombia/Project/Experiments/Aliivibrio and Streptomyces

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Contents

Aim

Figure 2. Model for the regulation of the chitinolytic system in S. coelicolor. (a) Repression and (b) induction of the chitinolytic system in S. coelicolor in the presence of related metabolic products of chitin. Read more in: Colson et al., 2003
Our system heavily relies in the ability of our bacteria to sense environmental cues belonging to the pathogens we’re particularly concerned of.
Figure 1. Model for Vibrio chitin utilization. A: Negative phenotype for chitin sensing. B: Positive phenotype. Activation of chitinolytic system due to presece of dimers of N-acetylglucosamine (GlcNAc)2 Read more in: Li and Roseman, 2003
Chitin is one of the main components of fungal cell walls and, in consequence, we will use it as an indicator of fungal infection.



Vibrio fischeri ES114 ([http://ijs.sgmjournals.org/content/57/12/2823 now Aliivibrio fischeri ES114]) and Streptomyces coelicolor A3(2) are environmental bacteria with well-characterized detection and catabolic cascades for chitin use as a carbon source. The way by which each bacteria detect the chitin is through a two-component system. Figure 1 illustrates the proposed sensing model for the system in A. fischeri, whose activation depends on the interaction CBP + N-acetyl-glucosamine starting the signaling from ChiS histidine kinase to turn on the chitinolytic genes. Figure 2 depicts how S. coelicolor uses a pretty similar system to the previously described, to phosphorylate ChiR regulator and induce transcription of the catabolism genes. In both cases, we expect to extract and put the genes into functional and independent biobricks available to further applications of chitin sensing and/or degradation.

Our bacteria

Aliivibrio fischeri ES114

Aliivibrio fischeri ES114
Taxonomy[1] Superkingdom Bacteria
Phylum Proteobacteria
Class Gammaproteobacteria
Order Vibrionales
Family Vibrionaceae
Genus Aliivibrio
Species A. fischeri
Strain ES114

Streptomyces coelicolor A3(2)

Streptomyces coelicolor A3(2)
Taxonomy[2] Superkingdom Bacteria
Phylum Actinobacteria
Class Actinobacteria
Order Actinomycetales
Family Streptomycetaceae
Genus Streptomyces
Species Streptomyces coelicolor
Strain A3(2)

References

[1] [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=312309&lvl=3&lin=f&keep=1&srchmode=1&unlock NCBI Taxonomy Browser: Aliivibrio fischeri ES114]

[2] [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=312309&lvl=3&lin=f&keep=1&srchmode=1&unlock NCBI Taxonomy Browser: Streptomyces coelicolor A3(2)]

[3] [http://www.pnas.org/content/101/2/627 Li, X., and Roseman, S. (2004). The chitinolytic cascade in Vibrios is regulated by chitin oligosaccharides and a two-component chitin catabolic sensor/kinase. PNAS 101, 627–631.]

[4] [http://mic.sgmjournals.org/content/154/2/373.full Colson, S., Wezel, G.P. van, Craig, M., Noens, E.E.E., Nothaft, H., Mommaas, A.M., Titgemeyer, F., Joris, B., and Rigali, S. (2008). The chitobiose-binding protein, DasA, acts as a link between chitin utilization and morphogenesis in Streptomyces coelicolor. Microbiology 154, 373–382.]

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