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Team:Caltech/Notebook/Biofuel
From 2012.igem.org
Biofuel Notebook Calendar
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Biofuel Notebook6/28/12We ran a test of the Ethanol Assay to determine its range of effectiveness. Back to Biofuel Notebook Calendar 7/3/12 We ordered materials for the ethanol assay and set up experiments to grow cells anaerobically to see if ethanol could be detected. Back to Biofuel Notebook Calendar 7/9/12 We started an aerobic and an anaerobic liquid culture of E. coli cells so that we could measure ethanol secreted the next day. Back to Biofuel Notebook Calendar 7/10/12 We used a plate reader to measure the ethanol concentrations but found both anaerobic and aerobic cultures produced elevated ethanol levels. This could have been because the cells grew too quickly and many were forced into anaerobic conditions in the aerobic culture. The OD (optical density) level of the aerobic culture was elevated. Back to Biofuel Notebook Calendar 7/13/12 We decided to try a few new ways to decrease aerobic ethanol yields and decided to try the experiment in flasks in a shaking incubator. Back to Biofuel Notebook Calendar 7/17/12 We made additional MOPS buffer and then set our aerobic culture experiment to incubate in a shaker overnight. Back to Biofuel Notebook Calendar this is h1this is h2>this is h3this is h4 |
