Monday, July 16
We ran multiple digests today, following the standard biobrick assembly protocol.
The gel of these digests can be shown below. [INSERT GEL PICTURE HERE]
In addition, we ran several PCRs today. Following the NEB protocol, we ran four PCRs to amplify the CCD plasmid (PUT PROPER NAME HERE) and also tried four colony PCRs, using colonies 2, 4, 13 and ______.
To enable amplification of our plasmid PSL2131, PsbA2, and CS42S, we designed primers to multiply the aforementioned DNA pieces. Tuesday, July 17
Wednesday, July 18
Thursday, July 19
Friday, July 20
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