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From 2012.igem.org

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Project Abstract

MiCodes (microCodes) are a system of cell barcodes that allow one to determine a yeast cell's genotype from its individual fluorescent phenotype. By localizing fluorescent proteins to distinct organelles within the cell, each yeast variant displays a unique pattern of colors. As the number of colors and target organelles raises, an exponential increase is found in the possible number of distinct barcodes. An extensive number of cells can be visualized at once by exciting the fluorescent proteins using fluorescence microscopy. Through computer or visual selection, one can sift through each combinatorial pattern of colors and targeted locations to identify a desired phenotype and elucidate the associated genotype.

The Micodes Library

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Application: Leucine Zippers

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