From 2012.igem.org
Monday
Scandal in the lab! It appears that someone at Caltech has edited our wiki, inserting the word 'chicken' into our project page. Have we been the victim of a not-so-droll ivy league-ish prank? We've emailed their project supervisor, so hopefully it shouldn't happen again.
One night seems to have been too little time to accurately determine if the transformation worked, as we now have lots of e.coli colonies on their plates. The bacillus plates have not been so successful, but the plasmid was not optimized to bacillus, potentially explaining their failure. We will need to make sure that we include more controls in future to work out where our experiments fail, if they should fail.
In any case, we were able to induce the pBAD promoter with arabinose in the e.coli cells. Results can be seen in the lab book page.
Tuesday
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