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From 2012.igem.org

Tom/Emeraldo

AlsT PCR with Pfu and Taq polymerase at 60 °C with primers 150, 250, 300, 500 bp.

Arjan/Renske

D-Day: washed and scanned the slides! The slides were subsequently washed using the ArrayIt® Microarray Wash Station and dried by centrifuging (2 min, 2000 rpm, RT). Slides were scanned using a Genepix Professional 4200AL (Axon instruments, Union City CA USA) array scanner, using the Genepix Pro 6.1 software (Axon instruments), default settings for Cy3/Cy5.