From 2012.igem.org

The Journal

Chitinase

To determine which chitinase is going to be used, we screened chitinase from 3 different species since the chitinase of different Vibrio spp. was not suitable or the strain was found (Alivibrio fischeri ES114, Alivibrio fischeri M11):

- Arabidopsis thaliana

- Colletotrichum spp.

- Trichoderma spp.

For each one we obtained accesion numbers:

- Arabidopsis thaliana: [http://www.ncbi.nlm.nih.gov/nuccore/AY099810.2 AY099810.2]

- Colletotrichum spp. : [http://www.ncbi.nlm.nih.gov/nucest/GW342409.1 GW342409.1]

- Trichoderma spp.  : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2]

Each one was tested using codon usage for bacteria (Translation table 11) to determine if the chitinase could be used. All of the sequences were suitable for use.

We selected Colletotrichum spp. and Trichoderma spp. sequences to design primers.

Primers

- Colletotrichum spp. :

- Trichoderma spp.  :

Ralstonia Solanacearum

June 8

Today we have our first meeting! We basically talk about the Ralstonia detection system and make a little research to find a proper medium to growth Ralstonia. Here is a little sketch we made.

June 12

We decided to prepare Casamino acid-Peptone-Glucose (CPG) media, which is a rich medium and we also have all the components in the lab.

This is the recipe for 1L of CPG.