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Team:Cambridge/Week 3/Lab book
From 2012.igem.org
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Contents |
Monday
Making bacillus competent
Bacillus salts *10 made up:
- (NH4)2SO4: 20g
- KH2PO4 (anhydrous): 60g
- Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
- KH2PO4 60g
- Tri-sodium citrate 10g
- MgSO4•7H2O 2g
- Added distilled water to make 1000mL
- Autoclaved
Medium A base *10 made up:
- Yeast extract 10g
- Casamino acids 2g
- Added distilled water to make 900mL
- Autoclaved
bacillus strain 168 streaked out and grown on plate overnight.
Tuesday
Making bacillus competent
Wednesday
Making bacillus competent
Thursday
Test transformation of frozen bacillus stocks
Friday
Transformation of bacillus and e.coli with lux genes from 2010
Testing of the spectral properties of filters
Blue , Green and Red filters were tested in different wavelengths (400 nm - 600 nm) in a spectrophotometer. Excellent absorbance/emmitance results given by the blue and red filters near 580nm and 490nm respectively. 580nm is the wavelength where intensity of light is to be measured to identify the presence of mOrange. 490nm is the wavelength where maximum light intensity is expected with or without the presence of mOrange.
