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From 2012.igem.org

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Diary	Lab book

Contents 1 Monday 2 Tuesday 3 Wednesday 4 Thursday 5 Friday

Monday

Making bacillus competent

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Bacillus salts *10 made up:

• (NH4)2SO4: 20g
• KH2PO4 (anhydrous): 60g
• Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
• KH2PO4 60g
• Tri-sodium citrate 10g
• MgSO4•7H2O 2g
• Added distilled water to make 1000mL
• Autoclaved

Medium A base *10 made up:

• Yeast extract 10g
• Casamino acids 2g
• Added distilled water to make 900mL
• Autoclaved

bacillus strain 168 streaked out and grown on plate overnight.

Tuesday

Making bacillus competent

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Wednesday

Making bacillus competent

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Thursday

Test transformation of frozen bacillus stocks

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Friday

Transformation of bacillus and e.coli with lux genes from 2010

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Testing of the spectral properties of filters

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