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From 2012.igem.org

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Diary	Lab book

Monday

Making bacillus competent

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Bacillus salts *10 made up:

• (NH4)2SO4: 20g
• KH2PO4 (anhydrous): 60g
• Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
• KH2PO4 60g
• Tri-sodium citrate 10g
• MgSO4•7H2O 2g
• Added distilled water to make 1000mL
• Autoclaved

Medium A base *10 made up:

• Yeast extract 10g
• Casamino acids 2g
• Added distilled water to make 900mL
• Autoclaved

bacillus strain 168 streaked out and grown on plate overnight.

Tuesday

Making bacillus competent

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