Team:Cambridge/Week 3/Lab book

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Diary Lab book


Monday

Making bacillus competent


Bacillus salts *10 made up:

  • (NH4)2SO4: 20g
  • KH2PO4 (anhydrous): 60g
  • Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
  • KH2PO4 60g
  • Tri-sodium citrate 10g
  • MgSO4•7H2O 2g
  • Added distilled water to make 1000mL
  • Autoclaved

Medium A base *10 made up:

  • Yeast extract 10g
  • Casamino acids 2g
  • Added distilled water to make 900mL
  • Autoclaved, then add :
  • 50g glucose, filter sterilized 100mL