Monday, July 9
Today, we maxi-prepped our construct CS42S, digested both CS42S and our plasmid PSL2131, and ran them both on gels. We made one-well gels in order to allow us to gel purify the constructs and recover DNA.
Then we gel purified the construct and plasmid and nanodropped them. [TABLE OF NANODROP RESULTS]
Tuesday, July 10 We learned that the biobrick protocol for ligation was not optimal for our conditions. Thus, using a different protocol [FOUND HERE PUT IN LINK], we performed a ligation of our construct CS42S and plasmid PSL2131. Then, we transformed some E. coli with the results of our ligation and put the plates in the incubator. In addition, as an extra check, we ran a gel of our ligation results to see if the procedure had worked. The gel reveals that we have several different ligations going on, including the ligation that we expect to see, at around 9 kb.
-LABEL THE GEL PICTURE WITH PROPER LABELS
Wednesday, July 11
Went to ylc miniprepped
Thursday, July 12
Digest CS42S and PSL2131 and the ligation from Tuesday that we grew up Gel purified the above Ligate the gel purification results Miniprep new parts + glycerol stock of new parts Started a maxiprep culture of zeaxanthin E. coli construct
Friday, July 13
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