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Team:Tec-Monterrey/allergen/project
From 2012.igem.org
Our project is based in the development of a standard production and expression of allergen proteins (from maize, dustmite and bees) in order for them to be easily purified and used in a new diagnostic kit designed for the detection of allergies.
The project involves the construction of an intron-engineered device that would allow the expression of allergen proteins in yeast, resulting in the secretion of the proteins to the medium, while also maintaining the ability to also produce the proteins in E. coli. The design of the kit is based on an ELISA assay and includes the production of modified scFv’s (single-chain variable fragment) that allow the detection of possible allergic reactions in an individual.
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Our project is based in the development of a standard production and expression of allergen proteins (from maize, dustmite and bees) in order for them to be easily purified and used in a new diagnostic kit designed for the detection of allergies. The project involves the construction of an intron-engineered device that would allow the expression of allergen proteins in yeast, resulting in the secretion of the proteins to the medium, while also maintaining the ability to also produce the proteins in E. coli. The design of the kit is based on an ELISA assay and includes the production of modified scFv’s (single-chain variable fragment) that allow the detection of possible allergic reactions in an individual. |
