Parts

Toxin- Antitoxin (TA) Modules

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We send all this parts to the Registry!!!!

[http://partsregistry.org/Part:BBa_K831000:Design HipA7] [http://partsregistry.org/Part:BBa_K831003:Design TisB] [http://partsregistry.org/Part:BBa_K831001:Design HipB] [http://partsregistry.org/Part:BBa_K831002:Design MqsA] [http://partsregistry.org/Part:BBa_K831004:Design istR ]

[http://partsregistry.org/Part:BBa_K831015 prm-hipA7] [http://partsregistry.org/Part:BBa_K831009 plac-hipB] [http://partsregistry.org/Part:BBa_K831007 ptetR-mqsR] [http://partsregistry.org/Part:BBa_K831014 plac-istR]

Ralstonia:

pchS: It encodes a histidine kinase sensor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME.

pchR: Receptor of a two-component signal transduction system. Responds to threshold concentrations of the Quorum Sensing unusual signal 3-OH PAME. It is an atypical response regulator that has a histidine kinase as its output domain. Autophosphorilation after 3OH-PAME recognition may have en effect in phcA activation.

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

[http://partsregistry.org/Part:BBa_K831016 PxpsR:] Promoter region of xpsR (PxpsR) wich integrates different signals including phcA. Expression of genes regulated by XpsR may be correlated with threshold concentrations of 3 OH-PAME.

[http://partsregistry.org/Part:BBa_K831017 PxpsR-eYFP:] Fluorescent Reporter to asses the response of PxpsR to different concentrations of 3OH-PAME.

Aliivibrio fischeri

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

chiP: [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

chiA: [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

chiS: [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.

cbp: [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

PChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

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