Team:Austin Texas/Week of July 2 to July 9
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Notebook for week of July 2 to July 9
Design of Converter Plasmid
- Razan
- 7/2 - Overlap PCR of Las Promoter and GFP+Terminator for control plasmid.
- 7/5 - Amplification PCR of gel extracted band from overlap PCR.
Design of Reporter Plasmid
- Peter
- 7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.