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Team:Queens Canada/Notebook/Week15
From 2012.igem.org
Control
Notebook - Week 15
| Date | Protocol | People | DNA (if relevant) | Quantities and Parameters (if relevant) |
|---|---|---|---|---|
| 08/10/2012 | Fast Digestion | Victor | ppFGFP + nFFGFP | |
| 08/10/2012 | Gel Extraction | Victor | nFFGFP + pFFGFP | |
| 08/10/2012 | Digestions | Kevin | J48200 w/ pSB1AT3 J13601 Lac operator w/ pSB1A3 746007 Antigen 43 w/ pSB1C3 | |
| 08/10/2012 | digestion | Faisal and David | SmtA and FMT with X and S | |
| 08/10/2012 | Gel Extraction | Phillip | IPTG (SP), T7 promotor (SP), mCerFC (1,3,4 - 2 trials each) | |
| 08/10/2012 | Digestion | Phillip | GE IPTG SP [X], GE T7 Prom SP [X] | |
| 08/10/2012 | Gel Electrophoresis | Phillip | 1. ladder 2. GE mCerFC 1,2 3. GE mCerFC 1,1 4. GE mCerFC 3,1 5. GE mCerFC 3,2 6. GE mCerFC 4,1 7. GE mCerFC 4,2 8. DIG GE DIG IPTG SP [X] T1 9. DIG GE DIG IPTG SP [X] T2 10. ladder 11. DIG GE DIG T7 Prom SP [X] T1 12. DIG GE DIG T7 Prom SP [X] T1 13. DIG nFFGFP XP 14. DIG nFFGFP 2 XP 15. DIG I15601 (pSB1A3) XP 16. DIG J45200 Banana Odour XP (pSB1AT3) 17. DIG 346002 Antigen 43 XP (pSB1C3) 18. Ladder | |
| 08/10/2012 | Fast Digestion | Victor | nFFGFP GE 2, ppFGFP GE 1, MPP Bio-timer k088006, MPP I13601, MPP Bis0A K123000, MPP Antigen 43 K346007 | denatured for 14 mins at 37 degrees. Heat inactivated at 80 degrees for 20 mins |
| 08/10/2012 | Gel Extraction | Victor and Kevin | 1. ladder 2. nFFGFP DIG XP 3. ppFGFP DIG XP 4. DIG Bio-timer k088006 XP 5. MPP Bis0A K123000 XP T2 6. DIG Antigen 43 K346007 XP 7. DIG KI13601 XP | |
| 08/10/2012 | PCR | Phillip | nFFGFP 1+2, ppFGFP 1+2 | 25 cycles, 2ul of dna used |
| 08/10/2012 | PCR | Beini | mCerFC | 25 cycles, 10uL of dna used |
| 08/10/2012 | Gel | Kevin | 1. ladder 7. J04500 SP dig 8. nFFGFP dig Xp | |
| 08/10/2012 | Digestion | Phillip | J04500 SP | |
| 08/10/2012 | Digestion | Beini | GE nFFGFP 1 [XP] | |
| 08/10/2012 | Gel | Beini | 1C3 XP GE, 1A3 BisdA XP GE, I13601 1A2 XP GE, J04500 SP GE, K088006 Timer XP GE pSB1A2, GE pFF GFP XP | |
| 08/10/2012 | Gel | Beini | mCer FC C, nFF GFP C, T7 prom GE X, IPTG SP GE, DIG GE IPTG SP X, GE T7 prom SP | |
| 08/10//2012 | Gel extraction | Beini, Kevin | mCer FC, pFF GFP, nFF GFP GE XP, pFF GFP XS | loaded 4 uL loading dye : 16 uL DNA; for digests with FD green, loaded all 30 uL |
Given that the vast majority of our work now is in the lab, we will only be updating the "Labwork" section of the Notebook.
