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Team:Lyon-INSA/BiofilmK
From 2012.igem.org
The INSA-Lyon iGEM 2012 solution: “Biofilm Killer”
Biological solutions presented above are very interesting and promising. But synthetic biology could make them even more powerful by providing a technology:
- able to control, prevent and protect industrial equipment
- using non-persistent molecules in the environment
- minimizing harm to individual, products and environment
To meet these challenges, we have chosen to build a bacterial strain based on the environmental friendly Bacillus subtilis strain (already used to feed animals and promote healthy vegetable growth. “Biofilm Killer” was engineered to both destroy and then replace, if needed, the deleterious contamination by a positive biofilm. Our solution is based on 3 genetic modules:
- KILL and SCATTER the biofillm. The effect of the biocide and scattering agents produced by the bacteria can be enhanced by the “torpedo” behavior of the Bacillus swimmers.
- COAT the surface with a surfactant reagent in presence of the inducteur 1 (xylose).
- STICK to install a positif biofilm in presence of the inducteur 2 (IPTG).
Estimating the cost of the current industrial solutions
In order to compare our biological solution to the currently used ones, we first approached companies whose concerns were similar to ours : they gladly gave us some of their data related to the price of their sterilizing solution.
The first company that answered to our sollicitations was Gattefossé : they estimated that, considering the use of chemical reagents (€50 per use : 3 kg of each CIP 84 and 92, €15 in disinfectants) and of a filter (€100 to €300 per use depending on its size), the total cost of one wash of a 1000L tank was from €165 to €365 ($215 to $475)per sterilization.
The second one was Realco and their enzyme solution : their cost estimation was €480 ($625) for a 1500L tank per sterilization.
Biofilm Killer implementation
Main advantages
Lower cost : No need to have a protein purification step which is very expensive.
Eco-frendly: “Biofilm Killer” won’t release toxic chemicals in the environment since the proteins used can be easily destroyed.
The “biological swiss-knife”: “Biofilm Killer” should be able to replace mechanical cleaning actions thanks to the swarming properties of our strain which can penetrate deep inside the biofilm and release the active molecules in situ all over the biofilm.
