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Team:Berkeley/Project/Localization/PromoterLibrary
From 2012.igem.org
Revision as of 18:14, 26 October 2012 by
Cheung.celia
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Narrow pixel brightness range (25-1000):
Vacuolar Membrane
Actin
Plasma Membrane
Nucleus
pTDH3
This strain of yeast cells did not grow; the over expression of the protein killed the cells.
The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter.
pTEF1
This strain of yeast cells did not grow; the over expression of the protein killed the cells.
pRPL18B
pRNR2
pREV1
Wide pixel brightness range (0-4095):
Vacuolar Membrane
Actin
Plasma Membrane
Nucleus
pTDH3
This strain of yeast cells did not grow; the over expression of the protein killed the cells.
The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter.
pTEF1
This strain of yeast cells did not grow; the over expression of the protein killed the cells.
pRPL18B
pRNR2
pREV1