Team:Trieste/notebooklast

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Week 11

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Suicide System

We tried to complete the construct, cloning the LL 37 construct inside the Holin ones, but unfortunately we didn't obtain positive colonies.

Antibody

The hybridoma producing a new neutralizing scFv against norovirus arrived. We extracted its mRNA and recuperated its cDNA. We cloned VL and VH fragments into a sequencing plasmid and now we are waiting to the sequences to arrive. Our hope is to compare two scFv and determine which binds better norovirus VLPs.

Cumate-Switch Regulation

CymR
We tried several times to clone the single J23100-CymR-B0015 Eco/Pst into the plasmid K510012 but we didn't have positive colonies.
Team iGEM 2012

Contact us

For other information, write to:

igem2012@gmail.com
Università degli studi di Trieste ICGEB Illy Fondazione Cassa di Risparmio
iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012
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