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Citrobacter freundii Characterisation:
Genome sequencing
One major advantage of E. coli over C. freundii is the fact that there is a lot of sequence information available on the former, while no whole genome sequences exist for the latter. In order to get the ball rolling, the genomes of two C. freundii strains (the type strain, ATCC 8090 and another strain our lab had, called SBS 197) were sequenced in Newcastle by Drs Wendy Smith and Anil Wipat with IonTorrent Sequencing.
To the best of our knowledge, no other iGEM projects have yielded genome sequencing data before, so this is a first both for our team, Europe and iGEM in general.
While a complete assembly of the sequence reads could not be done, these sequences constitute the first step towards unraveling the genome of our proposed chassis organism and even in this form they can provide valuable information.
The raw sequencing files (zip files) and the contigs that have been assembled de novo (.fa format) can be accessed at the public Dropbox folder
Sequencing Files along with pdf files of the assembly reports. In addition, the automated annotation spread sheets (done by RAST) for both strains can also be accessed from this location.
